Commercial Production of Nematode Antagonistic bio-agents TNAU Agri Notes PDF DOWNLOAD ECONTENT 2021

 NEM 451 Commercial Production of Nematode Antagonistic bio-agents (1+1)

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Lecture 1 Introduction – Economic yield loss caused by plant parasitic nematodes in agricultural and horticultural crops, Concepts and definition of bio control agents

Lecture 2 Types of Bio agents – Fungal antagonistic organisms – applications – dosage - commercial formulations available in India – Merits and demerits

Lecture 3 Purpureocillium lilacinum (=Paecilomyces lilacinus) – Morphological identification of colony, phialids and conidial spore

Lecture 4 Pochonia chlamydosporia – Morphological identification of colony, conidia spore and chlamydospores identification

Lecture 5 Isolation of P. lilacinum and Pochonia chlamydosporia from nematode eggs and mode of action

Lecture 6 Host range of P. lilacinum and P. chlamydosporia – virulence and effect of biotic and abiotic 

factors on growth – compatability with chemical inputs

Lecture 7 Sterilization – Types and Preparation of sterilizing agents – Equipments used for production of 

bioagents

Lecture 8 Principle of Autoclave, Laminar Air Flow Chamber and Fermentor

Lecture 9 Laboratory up-keep – Preparation of cleaning solutions – preparation of stock solutions

Lecture 10 Hemocytometer – Assessment of spore load – colony forming units

Lecture 11 Preparation of different culture media – Maintenance of pure culture - Mother culture – Sub culturing

Lecture 12 Mass culturing Techniques – Solid and liquid formulations – Shelf life

Lecture 13 Commercial formulations – Carrier materials – Packing – Quality control and shelf life

Lecture 14 Market demand analysis – Economics of Bio nematicides

Lecture 15 Establishment of pilot plant – Infrastructure requirement – cost analysis

Lecture 16 Budget preparation – marketing and cost – benefit analysis – risk analysis

Lecture 17 Environmental impact test with vertebrate and invertebrate organisms

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